Journal: iScience

Article Title: Discovery of a novel SHIP1 agonist that promotes degradation of lipid-laden phagocytic cargo by microglia

doi: 10.1016/j.isci.2022.104170

Figure Lengend Snippet:

Article Snippet: 5’PtdIns(3,4,5)P3 Phophatase Activity Fluorescence Polarization Assay , Echelon Biosciences , Cat# K-1400.

Techniques: Virus, Recombinant, Plasmid Preparation, Activity Assay, Fluorescence, Protein Extraction, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Transfection, Negative Control, In Vitro, Generated, Stable Transfection, Expressing, Software, Imaging, Selection

Journal:

Article Title: Mycobacterial Glycolipid Cord Factor Trehalose 6,6′-Dimycolate Causes a Decrease in Serum Cortisol during the Granulomatous Response

doi: 10.1159/000069971

Figure Lengend Snippet: Relative serum cortisol levels following TDM bead administration. C57BL/6 (solid symbols) and A/J (open symbols) mice were bled on days 0, 1, 3, 5, 7, 10 or 14 after intravenous injection with 105 TDM beads (left panel), or through to day 10 using 105 uncoated beads (right panel). Cortisol was measured by fluorescence polarization immunoassay. Assay sensitivity was 0.6 μg/dl (95% confidence). Data are expressed as mean values for 3 or 4 mice with standard errors shown.

Article Snippet: Cortisol was measured in an Abbott TDX/FLX analyzer using a fluorescence polarization immunoassay (Abbott Laboratories, Abbott Park, Ill., USA) with an assay sensitivity of 0.64 μg/dl (95% confidence).

Techniques: Injection, Fluorescence